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FASTQ files were adapter- and quality-end-trimmed using Trimmomatic

Note that the filenames, while ugly, are conveniently structured with the history of what you’ve done. This is a good idea.To run it on all of the pe qc files, do :

Well, first, you can get rid of original data. You already have it on a disk somewhere, right?

The offers a nice search utility page to obtain such information that was used extract selected columns. You can get data annotation for any SRA/ENA project using the url as shown below. Typical and links for the first &aposSRP033351&apos sample &aposSRR1039508&apos are:

Once we have an idea of the quality of our raw data, it is time to trim away adapters and filter out poor quality score reads. To accomplish this task we will use .$ cd # this command takes us the home directory

Another function needed for data preprocessing is cutting adapters. When the sequenced DNA template is shorter than sequencing length, part of sequencing adapters may be contained in the output reads. In this case, the adapters should be error-tolerantly detected and removed. Some tools like Trimmomatic [] and Cutadapt [] can handle such tasks, but they usually require users to input the sequence of the adapters, which are usually not well known for the people doing data analysis. By searching the best over

Related links


data adapters sequence reads pair